Light Microscopy: Leica TCS SP8 Confocal and STED 3x

  SingHealth Advanced Bioimaging 20 College Drive, Academia, Level 10 The Ngee Ann Kongsi Discovery Tower Singapore 169856  

What it is:
The Leica TCS SP8 confocal and STED 3X is a high end confocal microscope with STED imaging capabilities.

Manufacturer: Leica Microsystems

Model Name: Leica TCS SP8 Confocal and STED 3x

Applications: Confocal laser microscope, spectral imaging, fast X,Y,Z scanning Super-resolution STED imaging

Instrument Overview: The integrated STED system meets the requirements of daily scientific research and provides fast, intuitive, and pure optical access to structural details beyond the diffraction limit. STED 3X broadens the scope of super-resolution microscopy by using the spectrum range of visible light. It can reach lateral (XY) resolution of ~50 nm and axial (Z) resolution of ~150nm. Gated detection improves resolution. White Light Laser (470-670 nm) allows any kind of fluorophore combination and multicolor application.

Technical features and specifications:
Objective lens: HC Plan 5x/0.15,Apo 10x/0.4 CS2,Apo 20x/0.7 CS2,Apo 40x/1.3 Oil CS2,Apo 100x/1.4 STED White
Filters: Eye only (Transmitted light, DAPI, GFP, RFP)
Laser lines: 405nm diode laser, WLL 470-670nm,592nm(STED depletion) and 660nm(STED depletion)
Confocal imaging: PMT 1,PMT 2,Hybrid detector (HyD) -gating option available, PMT TRANS
Software: LAS X and Leica Lightning

Key Contacts

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