Multiphoton Microscope: Leica SP8 DIVE

What is it?
A multiphoton microscope for deep tissue imaging with minimal photobleaching

Manufacturer: Leica SP8 Deep In Vivo Explore

Model Name: SP8 DIVE

Applications:
Multi-spectral deep tissue imaging at fast speed and high resolution, label free imaging (such as fluorescence life time imaging -FLIM, Harmonic Generation-SHG, THG) in cells, 3D cultures, organoids, thick tissue sections.

Instrument Overview:
The first spectrally tunable multiphoton microscope, equipped with 4Tune, non-descanned detection offering unlimited flexibility for multicolour experiments. Equipped with tunable vario beam expander for improved sample penetration depth and maximum resolution.

Technical features and specifications:
Objective lens: 10x/0.3, 25x/0.95, 63x/0.9 water immersion objectives and special objectives for clarity (25x/1.00) and range of RI (16x/0.8)
Detectors: Four spectrally tunable filter free non-descanned detectors with detection range of 400-800nm

Light sources:
IR Laser Insight X3 DUAL with a continuous beam tunable from 680-1300nm and a second fixed output beam at 1045 nm
Stage: Motorized XY stage and high-speed super Z galvo stage with adjustable increments of <1.5nm in full range or <0.5 nm in restricted range

Software: LAS X

Charges: User operated – $50/hour, Staff operated – $90/hour (one time training fee: $200 for 2 hr session)

Instrument Manufacturer URL: https://www.leica microsystems.com/products/confocal-microscopes/p/dive/