T-Lab, Mechanobiology Institute – NUS 5A Engineering Drive 1, Level 9/10/11 Singapore 117411
What it is:
The iLAS2 TIRF is a total internal reflection fluorescence microscope for imaging close to the coverslip surface in multiple dimensions (xyt in multiple channels).
Manufacturer: GATACA systems
Model Name: iLas2
Applications: Total Internal Reflection Fluorescence (TIRF) Microscopy, Photoactivation (PA) or Fluorescence recovery after photobleaching (FRAP)
Instrument Overview: The iLas2 system is a unique ring TIRF system with azimuthal averaging that provides illumination uniformity for TIRF/wide-field imaging. The fast response time of the motorized TIRF angle and dual camera setup allow for simultaneous multi-wavelength TIRF imaging with penetration depth adaptation. It has a dual laser illuminator for TIRF imaging and FRAP/FLIP/PA applications.
Technical features and specifications:
Objective lens: 20x/N.A.0.45, 40x/N.A.1.15 (water), 60x/N.A.1.45 (oil), 100x/N.A.1.49 (oil)
Filters: FITC, TRITC
Laser lines: 405nm, 491nm, 532nm, 561nm, 642nm
Dichroic: Quadband dichroic and bandpass filter (single camera), T585LP dichroic with 525/45 & 609/54 (dual camera)
Cameras: 2x Fusion BT (6.5µm/pixel)
Stage: Prior motorised XY Stage
Software: MetaMorph, iLAS
Instrument Manufacturer URL:
https://www.gataca-systems.com/products/gataca-products/ilas-2/
Detailed Specifications:
https://simba-mbi.com/olympus-ilas2-tirf
