What it is:
The LaVision Trim II Multiphoton (MP) Microscope combines both inverted and upright microscope with a multiphoton laser and scanhead.
Manufacturer: Olympus
Model Name: LaVision Trim II
Applications: Label-free imaging, 3D imaging, Invtravital imaging, FLIM, FLIM-FRET
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Instrument Overview: MP microscopy enables optical sectioning deeper into samples thanks to the long wavelengths of excitation light. MP also allows label free imaging, meaning the auto fluorescence and the second harmonic generation (SHG; e.g. collagen fibers) of the sample can be used for 3D imaging. The inverted orientation is ideal for 3D imaging of cells/organs/tissues. The upright microscope is ideal for intravital imaging. In addition this system has a FLIM X16 bioimaging detector for fluorescence lifetime (FLIM) based applications. The measurement of fluorophore lifetime is based on Time Correlated Single Photon Counting (TCSPC) method. This enables FLIM experiments and FLIM-FRET applications. This system is equipped with both conventional PMT and highly sensitive GaAsP detectors and a Dodt microscopy detector for acquisition of transmitted light images. Both the inverted and upright microscopes are enclosed in cage-incubators to provide temperature, humidity and CO2 control.
Technical features and specifications:
Objective lens: 20x (water), 25x (RI liquids 1.41-1.52), 60x (water)
Filters: Blue, Green, Red
Excition Source: X-Cite 120Q for upright microscope body; Nikon intenslight C-HGFI illuminator for inverted microscope body
Laser Line: 680-1050nm
Detectors: 2 PMT detectors, 2 GaAsp detectors mainly used for auto fluorescence imaging, Becker & Hickl FLIM detector for 2 photon fluorescence lifetime imaging
Stage:
Software: Impspector (LaVision Biotech)
Charges:
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Instrument Manufacturer URL: https://tinyurl.com/TriMScopeII
Detailed Specifications:
https://tinyurl.com/AMP-LVMP
