What it is:
The spinning disk confocal with 3D-FRAP system is a multi-functional microscope built on a motorized Nikon Eclipse Ti inverted microscope equipped with a stage-top incubator and CO2 control system. It is capable of multi-position 2D and 3D live cell imaging in both spinning disk confocal and widefield modes.
Manufacturer: Various
Model Name: Nikon TiE Body
Applications: 2D and 3D Live-cell imaging, Confocal microscopy
Instrument Overview:
The Yokogawa CSU22 confocal scanning unit on this microscope allows for spinning disk confocal imaging in two colours (green and red emission) and DIC, with images captured by a Photometrics Evolve EMCCD camera. An additional iLas 3D-FRAP module allows users to carry out high precision controlled photokinetic experiments (405/491/561 nm) during spinning disk imaging. Widefield fluorescence, DIC and phase contrast imaging can be done on this microscope system using the second detector, a Hamamatsu ORCA Flash4.0 sCMOS camera. For both widefield and spinning disk confocal modes, a piezo z-drive enables fast acquisition of z-stacks for 3D imaging, and the Nikon Perfect Focus System (PFS) ensures long-term timelapse imaging without z drift. Sample holders for 35 mm diameter dishes and chambered coverslips (LabTek II and ibidi) are available.
Technical features and specifications:
Objective lens: 10x, 20x, 40x (oil), 60x (oil)
Filters: DAPI, GFP, TRITC, mCherry, Cy5
Laser Lines: 405m (Photoactivation only), 491nm, 561nm
Cameras: Hamamatsu ORCA Flash4.0 v2 (Widefield), Photometrics Evolve EMCCD (Spinning disk)
Stage: Motorised
Software: Metamorph
Charges:
User operated: $40.20/hour, Staff operated: $96.80/hour
Instrument Manufacturer URL: NA
Detailed Specifications:
https://tinyurl.com/AMP-SpinningDisk3DFrap
