Equipment at ABIF SCELSE: Zeiss ELYRA PS.1 + LSM 780

  SCELSE, Nanyang Technological University 60 Nanyang Drive, SBS-01N-27 Singapore 637551

Zeiss ELYRA PS.1 + LSM 780
What is it? A setup combining the ELYRA system (SIM, PALM and TIRF) with a LSM 780 laser scanning confocal microscope.

Manufacturer: Carl Zeiss

Model Name: ELYRA PS.1 and LSM 780

Applications Structured illumination microscopy (SIM) in 3D, Single molecule localization microscopy (PALM, STORM, PAINT, …), Single particle tracking (SPT) and fluorescence correlation spectroscopy (FCS), Total internal reflection fluorescence (TIRF) microscopy and Hi-Lo mode imaging, Laser scanning confocal imaging, Fluorescence spectral imaging, Fluorescence recovery after photobleaching (FRAP)

Instrument Overview: This setup combines the ELYRA system (SIM, PALM and TIRF) with a LSM 780 laser scanning confocal microscope. Besides the super-resolution techniques for which ELYRA is primarily designed, ELYRA’s high end cameras can be used for widefield imaging applications requiring high sensitivity of detection such as single molecule tracking. The motorised stage allows multi-position time-lapse imaging or tile scans of large samples. A stage-top incubator is available upon request.

Technical features and specifications:

Objective lens: 10x,20x (dry),63x (water),40x,63x,100x (oil)

Filters: DAPI, FITC, TRITC, cy5

Light sources: Transmitted (halogen), Fluorescence (metal halide), Lasers (405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm, 642 nm)

Cameras: Andor iXon DU-897D (mono), pco.edge sCMOS (mono)
Detectors: 2 PMTs, 32-GaAsP PMT array, transmitted light PMT

Stage: Motorised

Software: ZEN black

Instrument Manufacturer URL: NA

Detailed Specifications:
https://www.nobic.sg/ABIF/CZ_ELYRA.html

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