Equipment at AOBIP CSB: Inverted Fluorescence Live Cell Microscope - AO7

  SCELSE, Nanyang Technological University 60 Nanyang Drive, SBS-01N-27 Singapore 637551

Inverted Fluorescence Live Cell Microscope – AO7
What is it? A motorised inverted fluorescence microscope optimised for live-cell imaging.

Manufacturer: Carl Zeiss

Model Name: Axio Observer 7

Applications Widefield epifluorescence, Transmitted light imaging (brightfield, phase contrast, DIC), Long-term live-cell imaging
Instrument Overview: This motorised inverted microscope features a fast and sensitive sCMOS camera, a colour camera for transmitted light, motorised stage allowing multi-position time-lapse imaging or tile scans of large samples; hardware autofocus ensures continuously stable focusing throughout time-lapse acquisition of arbitrary duration. The microscope is housed in a heated enclosure with carbon dioxide control.

Technical features and specifications:

Objective lens: 10x, 10x, 20x, 40x (dry), 40x, 63x (oil)

Filters: DAPI, BFP, GFP, YFP, cy3, dsRed, cy5

Light sources: Transmitted light (LED), Fluorescence (LED)

Cameras: Hamamatsu Orca Flash 4.0 sCMOS (mono), AxioCam 105 (colour)

Stage: Motorised

Software: ZEN blue

Instrument Manufacturer URL: NA

Detailed Specifications:
https://www.nobic.sg/AOBIP/InvertedLiveCellAO7.html

Key Contacts

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