Zeiss Fluorescence Widefield Upright (#02-25)

What it is: Upright fluorescence wide field microscope

Manufacturer: Zeiss/Hamamatsu

Model Name: Zeiss Axio Imager M2

Applications: Fluorescence wide field imaging

Instrument Overview: A wide field fluorescence microscope for upright use.

Objective lens:

Objective lens	Magnification/N.A	Working Distance (mm)	Immersion Medium	Phase Contrast	DIC
Plan NEOFLUAR	5x/0.15	13.6	Dry	No	No
Plan APOCHROMAT	10x/0.32	2	Dry	Yes	No
Plan APOCHROMAT	20x/0.75	0.61	Dry	No	Yes
Plan NEOFLUAR	40x/1.3	0.21	Oil	Yes	Yes
Plan APOCHROMAT	63x/1.4	0.19	Oil	No	Yes
Plan APOCHROMAT	100x/1.4	0.17	Oil	Yes	Yes

 

Filters:

Filter Set	Excitation	Dichroic	Emission	Suitable Dye
Fs01	365/12	395	397LP	DAPI
Fs38	470/40	495	525/50	GFP/FITC
XF175	560/55	600	645/75	Cy3
Fs26	600/50	645	685/50	Cy5

 

 

Light sources:

• Fluorescence lamp: HBO Mercury short-arc lamp

 

Cameras:

• Camera: Hamamatsu ORCA-Flash 4.0v2 C11440-22CU
  • 2048×2048 sCMOS camera

 

Stage: Manual XY stage, motorised Z adjustment.

 

Software: MetaMorph 7.10.1

 

Charges:

Please contact bioimaging@tll.org.sg for latest updated charges

 

Instrument Manufacturer URL:

• Microscope body: https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html
• Camera: (not available)

 

Detailed Specifications:

http://microscopy.tll.org.sg/pages/light_micro%20-%202fwfup.html