MicroLambda Spinning Disk Inverted (#02-26)

What it is: Inverted spinning disk microscope with super-resolution capabilities.

Manufacturer: MicroLambda/Nikon/Yokogawa/Gataca

Model Name: Nikon Eclipse Ti with Yokogawa CSU X1 spinning disk system and Gataca LiveSR.

Applications: Spinning disk microscopy, FRAP, Super-resolution

Instrument Overview: A custom spinning disk microscope solution with super-resolution and FRAP capabilities. This microscope is also able to take images in wide field mode without using a simulated wide field like laser scanning confocals.

Technical features and specifications:

 

Objective lens:

Objective lens	Magnification/N.A	Working Distance (mm)	Immersion Medium	DIC
Nikon Plan Fluor	10x/1.3	0.3	Dry	No
Nikon Plan APO VC	60x/1.4	0.13	Oil	Yes
Nikon Plan APO VC	100x/1.4	0.13	Oil	Yes

 

Filters:

• Confocal:
  • Quad filter DAPI/GFP/RFP/Cy5
  • Tri filter DAPI/YFP/Cy5
• Wide Field:
  • DAPI
  • GFP
  • RFP

 

Light sources:

• Lasers: 405nm, 488nm, 515nm, 561nm, 640nm
• Fluorescence lamp: Metal halide lamp

 

Cameras:

• Camera: Photometrics Prime 95B
  • High frame rate 95% QE 1.2k×1.2k sCMOS camera
  • Actual usable area: 1028×720

 

Superresolution module: Gataca LiveSR, maximum resolution 105nm

 

FRAP module: Gataca iLas Pulse system

 

Stage: Nikon stepper motor stage with Nikon Perfect Focus System (PFS) for long time lapses.

 

Incubator: Chamber incubator with temperature control

 

Software: MetaMorph 7.10.3

 

Charges:

Please contact bioimaging@tll.org.sg for latest updated charges

 

Instrument Manufacturer URL:

• Microscope body: https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti-series
• Camera: https://www.photometrics.com/products/prime-family/prime95b
• FRAP system: https://www.gataca-systems.com/products/gataca-products/ilas-pulse/

 

Detailed Specifications:

http://microscopy.tll.org.sg/pages/light_micro%20-%20MLSD.html