Zeiss Fluorescence Wide Field Inverted (#02-25)

 

What it is: Inverted fluorescence wide field microscope with temperature control

Manufacturer: Zeiss/Hamamatsu

Model Name: Zeiss Axio Observer 7

Applications: Fluorescence wide field imaging

Instrument Overview: A wide field fluorescence microscope for inverted use. It also has an enclosure that can be temperature controlled.

 

Objective lens:

Objective lens	Magnification/N.A	Working Distance (mm)	Immersion Medium	DIC
Plan NEOFLUAR	10x/0.32	5.5	Dry	No
Plan NEOFLUAR	20x/0.5	2.1	Dry	Ph2
C-APOCHROMAT	40x/1.2	0.28	Water	No
Plan NEOFLUAR	40x/1.3	0.21	Oil	No
Plan APOCHROMAT	63x/1.4	0.19	Oil	No
Plan APOCHROMAT	100x/1.4	0.17	Oil	Ph3

 

Filters:

Filter Set	Excitation	Dichroic	Emission	Suitable Dye
Fs01	365/12	395	397LP	Long pass DAPI
Fs49	G365	395	445/50	Band pass DAPI
Fs38	470/40	495	525/50	GFP/FITC
Fs20	546/12	560	575-640	TRITC

 

 

Light sources:

• Fluorescence lamp: HBO Mercury short-arc lamp

 

Cameras:

• Camera: Hamamatsu ORCA-Flash 4.0v3 C13440-20CU
  • 2048×2048 sCMOS camera

 

Stage: Märzhäuser stepper motor XY stage, motorised Z adjustment.

 

Software: MetaMorph 7.10.3

 

Charges:

Please contact bioimaging@tll.org.sg for latest updated charges

 

Instrument Manufacturer URL:

• Microscope body: https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html
• Camera: https://www.hamamatsu.com/us/en/product/cameras/cmos-cameras/C13440-20CU.html

 

Detailed Specifications:

http://microscopy.tll.org.sg/pages/light_micro%20-%202fwfi.html