Leica SP8 Inverted gSTED (#02-19)

What it is: Inverted Leica SP8X gSTED laser scanning confocal microscope for high sensitivity and special fluorescence needs.

Manufacturer: Leica

Model Name: Leica SP8X gSTED

Applications: Laser scanning confocal microscopy, Stimulated Emission Depletion super-resolution imaging, simulated bright field.

Instrument Overview: A high sensitivity laser scanning confocal with a fully tunable laser light source for special fluorescence needs and a gating function to mitigate autofluorescence.

 

Technical features and specifications:

 

Objective lens:

Objective lens	Magnification/N.A	Working Distance (mm)	Immersion Medium	DIC
HC PL APO	10x/0.40	2.74	Dry	Yes
HC PL APO	20x/0.75	0.62	Dry	Yes
HCX PL APO	40x/1.3	0.24	Oil	Yes
HC PL APO	63x/1.4	0.14	Oil	Yes
HCX PL APO	63x/1.2	0.22	Water	Yes
HCX PL APO	100x/1.4	0.13	Oil	Yes

 

Filters:

• Confocal:
  • AOBS (Acousto-Optic Beam Splitter) spectral filter
• Ocular:
  • DAPI – DAPI filter
  • GFP – Green fluorescence protein filter
  • mCherry – mCherry/Red fluorescence protein filter

 

Light sources:

• Diode laser, constant wave: 405nm
• Argon lasers, constant wave (deprecated): 458, 476, 488, 496, 514nm
• White light laser, pulsed: 470nm – 670nm, continuously tunable.
• STED laser, pulsed: 596nm
• Fluorescence lamp: Metal halide lamp

 

Cameras:

• Confocal imager: 2x Hybrid Detector (high sensitivity avalanche diode detector), 2x Photomultiplier Tube detector.
• Transmitted light imager: 1x Photomultiplier tube

 

Stage: Motorised XY stage, High precision Galvo-Z stage.

 

Software: Leica LAS X

 

Charges:

Please contact bioimaging@tll.org.sg for latest updated charges

 

Instrument Manufacturer URL:

https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp8-sted-one/

 

Detailed Specifications:

http://microscopy.tll.org.sg/pages/light_micro%20-%20SP8.html