Leica SPE II (#02-19)

What it is: Upright Leica SPE II microscope for basic confocal microscopy.

Manufacturer: Leica

Model Name: Leica SPE II

Applications: Laser scanning confocal microscopy, simulated bright field.

Instrument Overview: A basic laser scanning confocal with a single PMT system for simple confocal imaging.

 

Technical features and specifications:

 

Objective lens:

Objective lens	Magnification/N.A	Working Distance (mm)	Immersion Medium	DIC
Leica ACS APO	10x/0.3	3	Dry	Yes
Leica ACS APO	20x/0.70	0.17-0.26	Oil/Glycerol/Water	Yes
Leica ACS APO	40x/1.15	0.27	Oil	Yes
Leica ACS APO	63x/1.30	0.16	Oil	Yes

 

Filters:

• Confocal:
  • Spectral filter
• Ocular:
  • A4ET – UV excitation, long-pass blue filter for DAPI viewing
  • I3 – Blue excitation, long-pass green filter for Green fluorescence protein viewing
  • 1 – Green excitation, long-pass red filter for mCherry/Red fluorescence protein viewing

Light sources:

• Diode laser, constant wave: 405nm, 488nm, 561nm, 633nm
• Fluorescence lamp: Metal halide lamp

 

Cameras:

• Confocal imager: 1x Photomultiplier Tube detector
• Transmitted light imager: 1x Photomultiplier tube

 

Stage: High precision Galvo-Z stage.

 

Software: Leica LAS AF

 

Charges:

Please contact bioimaging@tll.org.sg for latest updated charges

 

Instrument Manufacturer URL:

https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-spe/

 

Detailed Specifications:

http://microscopy.tll.org.sg/pages/light_micro%20-%20SPE2.html