Lightsheet: Bruker Invi SPIM Lattice Pro

  NUS Centre for Bioimaging Sciences, National University of Singapore Block S1A, Level 2, Lee Wee Kheng Building 14 Science Drive 4 Singapore 117557    

Bruker Invi SPIM Lattice Pro
The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams, or optical lattices.

Manufacturer: Bruker

Model Name: Invi SPIM Lattice Pro.

Applications: Standard and super resolution Fluorescence microscopy, Fluorescence correlation spectroscopy (FCS)

Instrument Overview:  InviSPIM Lattice Pro allows users to select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples.  its magnification changer provides 31.3x and 62.5x total magnification to allow user to optimize field of view and pixel size to the experimental needs as well. This system is recommended for live cell imaging and high speed imaging, as well as FCS for studying the photo physics of  fluorescence and diffusion behaviour and absolute concentrations of detected particles.

Technical features and specifications:

Objective lens: 28.6x 0.7 NA water immersion lens for illumination and Nikon CFI Apo 25x W 1.1 NA water immersion objective for detection. Total magnification: 31.1x and 62.5x.

Filters: Emission filters (camera short): Camera 1: BP 418–462; LP498; BP 500–530; BP 526–564; LP572; BP580–627; BP655–704. Camera 2: LP 498; BP 500–530; BP 526–564; LP 572;  BP 580–627; LP 656; BP 655–704. Dichroic mirrors: LP 499; LP 560.

Light sources: Diode lasers (nm): 405nm 100mW, 488nm 100mW, 515nm 100mW, 561nm 120mW (diode-pumped solid-state), 642nm 170mW. Laser safety class 1.

Cameras: Hamamatsu ORCA-Flash4.0v3 sCMOS, x2

Stage: invi SPIM sample stage moving in 3 axis

Software:  LuxBundle

Charges:

User operated: In office hours: $30/hour, After office hours: $15/hour.

 

Instrument Manufacturer URL:

https://www.bruker.com/en/products-and-solutions/fluorescence-microscopy/light-sheet-microscopes/invi-spim-lattice-pro.html

 

Detailed Specifications:

CBIS LM Core: InVi SPIM Lattice Pro – NUS Biological Sciences | NUS Biological Sciences

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