NUS Centre for Bioimaging Sciences, National University of Singapore Block S1A, Level 2, Lee Wee Kheng Building 14 Science Drive 4 Singapore 117557
Leica STELLARIS 8 FALCON
The STELLARIS 8 FALCON is an upright confocal point scanning system, using a White Light Laser with pulse picker as excitation light source and a highly sensitive, prism-based spectral detection with adjustable bandwidth, to offer flexibility in both choosing excitation wavelength and defining emission range. The FALCON (FAst Lifetime CONtrast) module is a fully integrated solution for Fluorescence Lifetime Imaging (FLIM) and enables video-rate lifetime imaging acquisition for rapid kinetic studies in live cells.
Manufacturer: Leica
Model Name: STELLARIS 8 FALCON (upright)
Application: Confocal point scanning system with DIC (20x and 63x) for live or fixed sample, FLIM.
Instrument Overview: A Confocal system has excitation and emission spectral imaging capability on top of normal laser scanning imaging. Additionally, the FAlCON allows user to follow fast molecular interaction, detect changes in metabolic state and microenvironment, determine lifetime and separate multiple fluorophores. This system is recommended for normal and high speed confocal 2D, 3D and stage imaging, to separate fluorescent signals and background, such as autofluorescence signal.
Technical features and specifications:
Objective lens: 10x/0.4 dry, 20x/0.75 dry, 20x/0.7 Imm, 20x/1.0 dipping, 63x/1.2 water, 63x/0.9 dipping, 100x/1.4 oil. With one single position nosepiece and one six-position nosepiece.
Filters: spectral detector
Light sources: 405nm (50mW), White Laser light (WLL, 440nm-790nm) (Laser power per line: 440 nm > 1.1 mW; 488 nm > 1.6 mW; 560 nm > 2.0 mW; 630nm > 2.6 mW; 790 nm > 3.5 mW).
Detectors: Power HyD S x2, HyD X x2, TPMT x 1
Stage: Scanning stage CFS flat (for DIC and stage stitching) or, AFM manual stage (with or without stage cover).
Software: LAS X STELLARIS
Charges:
User operated: In office hours: $30/hour, After office hours: $15/hour.
Instrument Manufacturer URL:
https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8/
Detailed Specifications:
CBIS LM Core: Leica STELLARIS 8 FALCON (upright) – NUS Biological Sciences | NUS Biological Sciences