Live-Cell Imaging: TIRF_1

  NUS Centre for Bioimaging Sciences, National University of Singapore Block S1A, Level 2, Lee Wee Kheng Building 14 Science Drive 4 Singapore 117557    

Total Internal Reflection Fluorescence Microscope (TIRF) _1
TIRF_1 is optimised for long term live cell TIRF imaging, to study membrane dynamics at the cell basal membrane, of actin, cellular adhesion, cell movement, vesicle, protein tracking, etc.

Manufacturer: GATACA Systems

Model Name: iLas 2 TIRF/FRAP

ApplicationsBrightfield microscopy, Total Internal Reflection Fluorescence Microscope (TIRF) with FRAP

Instrument Overview:Our TIRF_1 is equipped with iLas 2 TIRF/FRAP on Nikon Eclipse Ti inverted microscope, with Nikon Perfect Focus System 2.

Technical features and specifications:

Objective lens: 10X/0.3 dry Ph1, 60X/1.49 oil 0.13-0.21 DIC, 100X/1.49 oil 0.13-0.20, DIC

Filters: Dichroic mirror: Di01-R405/488/561/635; Emission Filters: 435/40, 520/35, 609/54, 692/40

Light sources: Diode laser: 405nm 100mW, 488nm 150mW, 561nm 100mW, 640nm 100mW; Transmitted (LED), Fluorescence (XCite 200DC mercury)

Cameras: Photometrics 95B back illuminated SCMOS (FOV19mm), air cooled.

Stage: Motorized

Software: Metamorph

Charges:
User operated: In office hours: $20/hour, After office hours: $10/hour

Instrument Manufacturer URL: https://www.gataca-systems.com/products/gataca-products/ilas-2/

Detailed Specifications:
https://www.dbs.nus.edu.sg/2021/03/12/cbis-lm-core-total-internal-reflection-fluorescence-microscope-tirf-_1/

Key Contacts

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